Histosonda®

A New Generation of Probes for In Situ Hibridization

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The Protocol

Frequently asked questions about the Histosonda® Protocol.

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1	How recently should my sections be cut?
2	How thick should I cut my tissue sections?
3	Can I use tissue sections fixed with B5?
4	Can I use less probe?
5	Why is it necessary to use a humid chamber?
6	How is the probe incubation period only 1 hour?
7	Why should the slides not be covered during hybridization?
8	Can I use PBS with Tween?
9	What is the decalcification method recommended by CENBIMO?
10	Can I use cover slips during the probe incubation if I wish?

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