Histosonda®

HistoSonda® is a Single Stranded DNA probes much longer that Oligo Probes.

HistoSonda® is a new Generation of Probes for In Situ Hybridization, a vast improvement on currently available technologies in Histopathology Labs.

Compared to Oligos, HistoSonda® is :

FASTER:The HistoSondas® have an incubation temperature of 62ºC. The elevated thermal energy generated allows the molecules to vibrate quicker therefore taking less time to find their target even though they are situated in a very viscose dextran containing media. The use of 62ºC as the incubation temperature for these probes therefore permits rapidity. HistoSonda® is the only commercial probe whose incubation period is just 30-60 minutes.

MORE SPECIFIC: Due to their great lengths the HistoSondas® are more specific. It is statistically less probable to find shared sequence homology between long sequences. Therefore the chances of the probe annealing with anything other than the target sequence are greatly reduced, increasing specificity. The HistoSonda® incubation temperature of 62ºC also permits that only chains with almost total sequence homology are annealed (an oligo will not anneal at this temperature).

MORE POTENT: The potency of the HistoSondas® is directly influenced by the number of labeled nucleotic in the DNA sequence. As the HistoSondas® have long sequences, the probability of large numbers of labeled uridines being incorporated is greatly increased, increasing the potency of the signal.

SIMPLE TO USE: The HistoSonda® hybridization procedure has been simplified to the maximum. It only uses one buffer during the entire technique (PBS). The use of formamide is not necessary and post-incubation washes with formamide have therefore been omitted. The hybridization procedure can be carried out without needing to have specific knowledge of Molecular Biology.

Complicated apparatus is not necessary and revealing the probe can be performed automatically using any immunohistochemistry apparatus.

EASY TO TRANSPORT AND STORE: The HistoSondas® are supplied Lyophilized in single use tubes. Transport and storage can be carried out at room temperature and they have a long storage life. Freezers, refrigerators or any special type of care are not required.